Analysis of ESTs from multiple Gossypium hirsutum tissues and identification of SSRs.

Generation of ESTs for Flowering Gene Discovery and SSR Marker Development in Upland Cotton

BACKGROUND Upland cotton, Gossypium hirsutum L., is one of the world's most important economic crops. In the absence of the entire genomic sequence, a large number of expressed sequence tag (EST) resources of upland cotton have been generated and used in several studies. However, information about the flower development of this species is rare. METHODOLOGY/PRINCIPAL FINDINGS To clarify the mo...

Genome-wide identification of membrane-bound fatty acid desaturase genes in Gossypium hirsutum and their expressions during abiotic stress

Membrane-bound fatty acid desaturases (FADs) are of great importance and play multiple roles in plant growth and development. In the present study, 39 full-length FAD genes, based on database searches, were identified in tetraploid upland cotton (Gossypium hirsutum L.) and were phylogenetically clustered into four subfamilies. Genomic localization revealed that 34 genes were mapped on 22 chromo...

Effect of Kinetin on Multiple Shoot Induction in Cotton (Gossypium hirsutum L.) cv. NIAB-999

A global assembly of cotton ESTs.

Approximately 185,000 Gossypium EST sequences comprising >94,800,000 nucleotides were amassed from 30 cDNA libraries constructed from a variety of tissues and organs under a range of conditions, including drought stress and pathogen challenges. These libraries were derived from allopolyploid cotton (Gossypium hirsutum; A(T) and D(T) genomes) as well as its two diploid progenitors, Gossypium arb...

In vitro Plant Regeneration from the Callus of Shoot Tips in Cotton (Gossypium hirsutum L. cv. SVPR 2)

A high efficiency shoot organogenesis from callus cultures and plant establishment protocol has been developed for cotton (Gossypium hirsutum L. cv SVPR-2) through shoot tip. After 6 weeks of culture, green-compact-nodular organogenic callus induction was observed on the medium fortified with (MS) basal salts, B5 vitamins, 2.0 mgl-1 of benzylaminopurine (BAP), 1.0 mgl-1 kinetin (KN), 30 gl-1 gl...